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Immunofluorescence analyses indicated that in control A549 cells or cells infected with Legionella SidEs deletion strain, VSVG reached its peak of accumulation in the Golgi after 20 min of incubation at 32 C, and the colocalization index then gradually decreased as the protein is trafficked from the Golgi to secretory vesicles

After several cycles of refinement and model building, the inhibitor was placed into the cryo-EM map