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2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

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PR-39 and PR-35 showed an IC50 value of 16 g.mL -1 , but PR-35 had a higher cell survival rate than PR-39 (52)