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Hatsukami DK, Hertsgaard LA, Vogel RI, Jensen JA, Murphy SE, Hecht SS et al (2013)

RNAScope FISH (tracheal digest cytospin labeling) Tracheal cells prepared for 10X scRNA-seq were distributed on slides via centrifuge at 55.3 g , fixed for 15 min in 3.2% PFA/1.5% sucrose at room temperature, rinsed with 1 PBS and stored at 4 C