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Lentiviruses were generated by transfecting HEK293T cells with shRNA or EV, envelope plasmid pMD2.G (#12259, Addgene, RRID: Addgene_12259) and the packaging vector psPAX2 (#12260, Addgene, RRID: Addgene_12260)

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Introduction Electronic cigarettes (e-cigarettes) use an electrical resistance coil to vaporize mixtures of propylene glycol (PG), glycerol (GL), nicotine, and flavor chemicals