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For this purpose, 20 l from mobile phase, Milli-Q water, placebo, NAC, Di-NAC standards and placebo sample solutions was injected into the HPLC system separately, and the chromatogram results are in Figure 3Specificity chromatograms.20 L of mobile phase (A) , placebo (B) , Milli-Q water (C) , NAC standard (D) , placebo sample (E) and Di-NAC standard were injected