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In athletic performance, mitochondrial efficiency directly influences endurance, recovery speed, and overall work capacity

In brief, cells were washed twice with phosphate-buffered saline and resuspended in prewarmed (37 C) Seahorse basal medium (Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 5 mM glucose (pH 7.4)

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It is not clear how DHA affects brain function, but changes in DHA content of neuronal cell membranes could alter the function of ion channels or membrane-associated receptors, as well as the availability of neurotransmitters (94)