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The amount of unencapsulated GHK-Cu which diffused into the solution was determined via HPLC (Agilent 1100, Agilent Technologies, Inc., Santa Clara, CA, USA) using a UV-Vis detector at a wavelength of 220 nm using a 0.2% trifluoroacetic acid solution (TFA) and the ODS C18 column (Keystone Scientific, Inc

doi: 10.1080/10715769900301451 25 GullnerG.ZechmannB.KnstlerA.KirlyL

After a period 3 months, zinc therapy alone or in combination with Vitamin E or Vitamin E + C was associated with significant improvement in sperm parameters and reduction in OS, sperm apoptosis, and SDF.[20] The results of this study are limited due to small sample size