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For transient expression of FOXO1, we transfected cells with pBabe-Puro-FOXO1(de Keizer et al., 2010)

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A 2018 review in the International Journal of Molecular Sciences (Pickart and Margolina) found the cosmetic anti-aging case for GHK-Cu rests more on cell-culture and mechanism studies, showing it stimulates collagen and elastin production in lab settings, than on large, face-specific clinical trials measuring actual wrinkle reduction in real people

A 15-year follow-up study published in 2011 found that patients with a rapidly aging cardiovascular system who received epithalamin treatment for 2-3 years experienced a 1.6-1.8 fold decreased mortality rate compared with the control group [12]