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Extracted peptides were then pooled in an ethanol-rinsed tube, vacuum dried and re-dissolved in 18 l 0.1% trifluoroacetate

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Confocal images of organoids and thick tissue sections were acquired on an inverted single-photon point scanning confocal microscope (Zeiss Cell Discoverer 7 with LSM 900 and Airyscan 2) using a Zeiss APOCHROMAT 20/0.95-NA Autocorr air objective, with a tube lens of 0.5 or 1, and a voxel size of 0.4 0.4 0.5 m or 0.5 0.5 0.5 m for organoids and 0.3 0.3 0.3 m for thick tissue sections

Without deliberate effort to preserve muscle, the resulting body composition after major weight loss can be disappointing despite excellent scale results