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To confirm that the gut microbes were indeed major regulators of weight gain in the smoking cessation mice, the researchers collected microbiomes from smoking or smoking-cessation mice at various timepoints, and transferred these into germ-free mice that had never been exposed to cigarette smoke

Others like the die-off of megafauna at the end of the last ice age may be directly linked to human behavior, and it's this detail that propels Elizabeth Kolbert's fantastic, Pulitzer-Prize winning nonfiction book The Sixth Extinction

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These urine tests detect Cotinine a by metabolite of Nicotine which is excreted reliably in urine