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Nazem A, Tang CC, Spetsieris PG, Dresel C, Gordon ML , Diehl-Schmid J, Grimmer T, Yakushev I, Mattis P, Ma Y, Dhawan V, Eidelberg DA

The protein pellet was resuspended in 100 L RIPA buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, and 140 mM NaCl) to normalize the intensity values for each targeted metabolite

High-fiber diets exert renal protective effects through multiple mechanisms: increasing the intestinal carbohydrate-to-protein fermentation ratio, thereby reducing production of uremic toxin precursors such as indole and p-cresol

Store in the refrigerator or freezer until ready to use