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Plasma amino acids Plasma amino acid concentrations were measured by ultra-high performance liquid chromatography (ACQUITY H-Class System, Waters Corporation, MA, USA) using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (Waters AccQ Tag assay kit, MA, USA) and norvaline (Sigma Aldrich, MO, USA) as internal standard as previously described 11