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Intramolecular ligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups
and has been tested on human
since any contaminating DNA would also be a template for random-primed cDNA synthesis
The enzyme is supplied with a 10X Reaction Buffer
Plasmid-Safe-ATP-Dependent DNase, 10,000 U @ 10 U/uL size:EACH Intramolecular ligation of single-stranded DNARemove contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC preps Digest contaminating linear DNA without digesting nicked or closed circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.) Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations Utilise whenever protecting circular dsDNA is important Plasmid Safe ATP Dependent DNase selectively removes contaminating bacterial
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