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Furthermore, the assay was adapted for high-throughput screening platforms, with the datasets coupled with structural modifications that were previously proposed to impact accumulation in E

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Any discoloration (yellowing, browning, or grey tint) suggests oxidative degradation or temperature excursion during shipping

The cells were incubated for 30 min at 37C, centrifuged for 3 min, washed 1X with PBS, and fixed with 4% formaldehyde in PBS for 30 min statically at room temperature