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Bacterial pellets were resuspended in GST-binding buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA) containing protease inhibitors

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Silent information regulator 1 (Sirt1), an anti-inflammatory and anti-oxidative protein (316), increases the production of NO by activating endothelial NOS (eNOS) in endothelial cells in response to shear stress (317)