From 1 mL of the initial soluble GST-GSH lysate associated with the PEGDA:GSH homogenates, 0.8 g were eluted from the PEGDA homogenates, and 15 g were eluted from the PEGDA:GSH homogenates
65 ) (Addgene, 52961) for CRISPRCas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41
At the same time: most peptide therapies discussed for injury recovery are still considered experimental and have not yet reached the level of evidence required for routine clinical use
In certain embodiments, the viscosity of the gel is in the range from about 100 to about 50,000 cP, about 100 cP to about 1,000 cP, about 500 cP to about 1500 cP, about 1000 cP to about 3000 cP, about 2000 cP to about 8,000 cP, about 4,000 cP to about 50,000 cP, about 10,000 cP to about 500,000 cP, about 15,000 cP to about 1,000,000 cP