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However, despite the mitochondrial release of cytochrome c [107,113] during APAP-induced liver injury, there is no activation of caspases [114,115] and all morphological characteristics of necrosis including cell and organelle swelling, cell contents release and karyolysis are evident both in vivo and in vitro [116-118]

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Studies have reported that feeds supplemented with Spirulina improve intestinal barrier biomarkers in fish and chickens and increase the diversity of healthy microorganism communities (84, 107109)