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Metformin inhibits beta-catenin phosphorylation on Ser-552 through an AMPK/PI3K/Akt pathway in colorectal cancer cells

At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)

Future mechanistic studies should use CVB-infected human islets to quantify the production, processing, and display of key autoantigens, utilizing proteomics or other methods, following infection-induced ER stress and comparing results to an inactivated virus as a control

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