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To reconstitute any peptide, GLP-1, or supporting compound such as NAD+, divide the total peptide amount in the vial (mg) by the amount of bacteriostatic (BAC) water added (mL) to find the concentration (mg/mL), then divide the desired dose (mg) by this concentration (mg/mL) to determine the injection volume in syringe units

doi:10.1016/j.maturitas.2016.04.006 225

Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA)