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For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)

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Pro-inflammatory cytokines, IL-1, TNF, and IL-6, contribute to tissue repair by enhancing phagocytic activity, excessive release leading to the upregulation of inducible nitric oxide synthase (iNOS), damaging neurons, and disrupting the bloodbrain barrierthe anti-inflammatory response results in neuroprotection by tissue repair, regeneration, and promoting angiogenesis (Figure 15)