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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

To address the heterogeneity of the included studies, we opted for a structured synthesis based on target populations (e.g., MCI, SCD, mixed samples) and outcome domains (e.g., cognitive, motor, usability)

Optimizing application technique When you apply matters