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All necessary precautions were taken to ensure the animals wellbeing and minimize any potential discomfort or pain

The sections were then incubated with FSB solution, and then soaked in lithium carbonate solution

Macronutrients include carbohydrates, proteins, and fats

On the other hand, in ischemia/reperfusion (I/R) model, it is well-known that ROS production and the subsequent secretion of TNF- by inflammatory cells significantly contributes to the injury, and preconditioning by short-term ischemia increases resistance to subsequent lethal ischemia/reperfusion with the suppression of massive inflammatory activation