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49 Additionally, we evaluated the association rate constants, k on , which are dominated by the diffusive properties of the partners and geometric constraints of the actual binding, for the three complexes by applying the expression, where N A is Avogadros number, V eff is the effective volume of a half-sphere with radius r eff around the tip, which describes the effective volume for proteins binding, and t 0.5 is the time required for the half-maximal binding probability

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