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Description

The resulting samples were crosslinked with a limiting amount of glutaraldehyde at 0.075% for 25 min at room temperature and quenched with 100 mM Tris-HCl pH 8.0 for 5 min

The PCR amplicon sizes for the 5 check and 3 check of GDH2 gene disruption, 490 and 980 bp, matched the predicted values of 466 and 950 bp, respectively