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A rat model of MI was used in this study and post-MI rats were randomly assigned into 5 subgroups ( n = 7/group): (1) vehicle (3%V/V DMSO), (2) enalapril (10 mg/kg), (3) zVAD-FMK (1 mg/kg), (4) Necrostatin-1 (1.65 mg/kg), or (5) Ferrostatin-1 (2 mg/kg)

Allow longer settling time: Larger batches benefit from 2448 hours of rest before bottling to allow air bubbles to fully escape