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Unlike the traditional colony-forming culture system (e.g., culturing iPSCs using E8 medium or mTeSR medium on Matrigel-coated tissue culture plates) (26), the DEF-CS culture system supports single-cell growth (no colony formation) during cell culture

Glutamine is an amino acid essential for many important homeostatic functions and for the optimal functioning of a number of tissues in the body, particularly the immune system and the gut

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