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Acute injuries typically require one complete cycle

This is why some practitioners prefer it not just for multi-dose use but specifically because it's designed to remain chemically stable in a vial over an extended period

It also plays a role in protecting B12 from being destroyed by stomach acid, such as in the stomach and intestines.[ref] TCN2 (transcobalamin II, TCN2 gene): TCN2 is a B12 transport protein that moves vitamin B12 from the bloodstream into cells throughout the body