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Methylene blue acts as a co-factor for nicotinamide adenine dinucleotide phosphate (NADPH)-dependent methemoglobin reductase, which leads to the formation of reduced methylthioninium chloride that can reduce ferric iron to ferrous iron needed to restore the oxygen-carrying capacity of hemoglobin

In general, semipolar compounds serve as intermediate solvents, eliminating polarity from nonpolar liquids and bringing them into miscibility

"If a drug has only been tested in younger people, then how do you know it's also going to be good [in someone who is older?], says Raymond Yung, M.D., a professor of geriatric medicine at the University of Michigan and associate director of research at the Department of Veterans Affairs Ann Arbor Geriatric Research Education and Clinical Center

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