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Disk state of TR-His-TMV-CP 19 in Solution In the size exclusion chromatography, the retention volume of N-His-TMV-CP 19 protein under 20 mM Sodium Phosphate buffer and 100 mM Sodium Chloride solution (PH 8.0) was the oligomeric state (dimmers and monomers), and the retention volume of TR-His-TMV-CP 19 was transformed to disks mostly after dialyzing against 0.20.3 mol/L ammonium sulfate and 0.1 mol/L Tris ( PH 8.0 ) solution at room temperature for more than 10 hr

B-scan ultrasonography to rule out retinal tear or detachment if unable to visualize the fundus