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Recent work has highlighted persistent challenges in metabolomics and ML-based biomarker discovery, including variability in sample preparation, platform-dependent effects, and the risk of overfitting in high-dimensional datasets 76,77

van Geldermalsen M, Wang Q, Nagarajah R, Marshall AD, Thoeng A, Gao D, et al

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The strongest human evidence is topical, including the Leyden 2002 photoaging trial and a 2023 IRB-approved 21-woman topical NEEL gel study reporting an average 28% increase in skin collagen density at 3 months