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Description

For severe deficiency, the AAFP recommends loading doses typically daily or every other day for one to two weeks followed by monthly maintenance injections

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At the higher 30-unit (6mg) dose, the vial lasts approximately 13 days

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To enable intracellular staining, cells were fixed with Pierce 1.6% formaldehyde (Thermo Fisher Scientific) in Maxpar PBS for 10 min at RT and washed twice with Maxpar Cell Staining Buffer

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