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In summary, accumulating evidence supports a critical role for ferroptosis in secondary injury after both TBI and SCI, driven by iron overload, lipid peroxidation, and dysregulated ferroptosis-associated proteins (114, 225235)

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RNA quality was examined by the 4200 TapeStation (Agilent Technologies, Santa Clara, CA, USA) and RNA concentration was determined using the Qubit Fluorometric Quantitation (Thermo Fisher Scientific)

But sedentary lifestyle, stress, environmental toxins, age, poor nutrition, & many other factors negatively impact glutathione levels