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However this idea didnt raise many researchers interest until 1968 with the discovery of na enzyme that is specific for the catalytic removal of na oxygen radical (MCCord and Fridovich, 1969)

The tissue was then blotted dry and weighed followed by homogenization in 200 l buffer (0.05 M potassium phosphate and 0.1 mM EDTA, pH 7.8) and centrifuged at 15,000 xg for 30 min at 4C