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Indeed, the tobacco industry was a state monopoly until 1985, and Japans tobacco control policies are considered to be weaker than those in other high-income countries.[v] There were also a range of societal and cultural factors that meant HTP might prove successful in the country

Cells were symmetrically bathed in a modified Ringers solution (120 mM NaCl, 10 mM d-Glucose, 3.3 mM KH 2 PO 4 , 0.83 mM K 2 HPO 4 , 1.2 mM MgCl 2 , 1.2 mM CaCl 2 , 25 mM NaHCO 3 , pH 7.4)