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Sequencing was carried out by Sanger methodology on ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and 100% concordance with restriction fragment length polymorphism assays was shown providing confidence in using the full dataset in subsequent analysis

Tremors, bradykinesia, and postural instability manifest following a 7080% depletion of dopaminergic neurons in the substantia nigra (SN) [3] and subsequently, dopamine release in the striatum is impaired [4]