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Name: weed smoking vs cigarettes E-Cigarettes (Vapes) | and Tobacco Use Explore smoking vs vaping cannabis
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However, it wasn't until the 1950swith the introduction of the legendary "Marlboro Man" campaignthat it transformed into the symbol of rugged masculinity and freedom we know today

At 80% confluence, cells were detached with 0.05% trypsinEDTA (Gibco) and seeded on membrane supports (12 mm Transwell culture inserts, 0.4 m pore size, Costar) coated with 0.05 mg collagen from calf skin (Sigma-Aldrich) in ready-to-use AEGM supplemented with 1% penicillin/streptomycin