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To the right are widefield images of HeLa cells with lentivirus mediated LUC, Lb NOX, Ec STH, mito Ec STH expression under Dox control transiently expressing iNAP1, in basal medium (DMEM without pyruvate, fluorescent vitamins and phenol red with 5 mM glucose, 25 mM HEPES, pH 7.4 and 1% dialyzed FBS), or when cells were switched to the basal medium without glucose but with 10 mM pyruvate

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If you need help transitioning from morning to evening dosing, check out this article, which walks you through it