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Immune cells from aged versus nonaged adult killifish display different functional responses to LPS stimulation and have increased markers of senescence To test whether the molecular and cellular changes we observed in the killifish kidney marrow during aging are associated with an impaired immune cells response in vitro, we adopted an assay that measures immune cell activation in the presence of LPS, a potent immune stimulator 54 (Fig

K.MaurinG.CouvreurP.et al (2012)

The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord

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