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This makes it possible to identify and analyze a significant and large number of metabolites, typically in the range of hundreds, that are of great value to interpret the most relevant alterations associated with the toxic insult of a particular compound ( Metabolomics has emerged, over the last 2 decades, as a mature tool in toxicology, enabling us to obtain information about the effects of xenobiotics on target organs and improving our understanding of the modes of action of bioactive/toxic compounds