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Equal amounts of total protein (30 g per sample) were resolved by 1012% SDS-PAGE and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore or Invitrogen, Shanghai, China)

The human GST multi-gene superfamily is encoding for various ubiquitous cytosolic or soluble, mitochondrial and microsomal as well as peroxisomal homo- and heterodimeric transferases (Di Pietro et al., 2010)

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Activated immune-inflammatory and oxidative and nitrosative stress (IO&NS) pathways may underpin the somatic and mental symptoms of Long COVID because chronic fatigue syndrome, major depression and generalized anxiety disorder (GAD) are characterized by activated IO&NS pathways