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Method Optimisation Varying amounts of O-pthaldialdehyde (OPA) reagent (110%, v / v ) (Sigma-Aldrich, Poole, UK) were titrated with 2 mM GSH to determine the optimal reactivity (product yield)

Likewise, SiO 2 NPs also enhanced chl b content, with the maximum amount of 7.001.07 g g -1 FW was recorded at 8 mg L -1 , while slightly higher amount was observed after treatment with 16 mg L -1 Si bulk (7.700.72 g g -1 FW)

The underlying biological phenomenon involves ROS, which play a complex dual role in sperm physiology and pathology

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