a,b , Representation of the stationary points calculated with ONIOM(B97X-D/6-311 + G(2d,p):ff14SB,gaff2,TIP3P) G(d):ff14SB,gaff2,TIP3P) for the Pt GSTF8-catalysed deprotonation-protonation reaction: flavan-3-on-4-ol ( 7 ), transition state for deprotonation ( 7-TS ), deprotonation product ( 8-enolate ) and cyanidin ( 8-B4 ) bound to the wild-type enzyme ( a ) and the C13S variant ( b )
To construct vectors for the Y2H assays, LET7 , LET7 TPR , LET7 PPase , HOP1 , HOP1 TPR1 (600 bp, from 1 to 600), HOP1 TPR1 / 2a (1,140 bp, from 1 to 1140), HOP1 TPR2a / 2b (1,116 bp, from 601 to 1716), HOP1 TPR2b (576 bp, from 1141 to 1716) or CRCK3 SP (1,440 bp, from 91 to 1530, without the signal peptide) were amplified via PCR from the pHBT -p35S :: LET7-HA , pHBT -p35S :: HOP1-HA or pHBT -p35S :: CRCK3-HA vectors and subcloned into the pGADT7 and pGBKT7 (Clontech) vectors
The 8-oxo-dG adduct in the mitochondrial and nuclear DNA is a pre-mutagenic lesion formed primarily by an attack by the hydroxyl radical ( OH)
Tackling the aging process with bio-molecules: a possible role for caloric restriction, food-derived nutrients, vitamins, amino acids, peptides, and minerals
