In this study, Sc GSH1, Sc GSH2, and St GshF were heterologously expressed in Pichia pastoris GS115 to realize the dual-path synthesis of GSH in yeast
Finally, the proteins were visualized by applying Super Signal West Femto Chemiluminescent Substrate (Thermo Scientific) to the membranes, followed by detection in the molecular imager ChemiDoc MP and quantification of the detected bands using the Image Lab software (Bio-Rad Laboratories)
Dosing usually starts around 600 mg twice daily
Enzyme-Linked Immunosorbent Assay Analysis Enzyme-Linked Immunosorbent Assay Analysis (ELISA) kits were used to measure the levels of P4HB, CDH13, C1QA, Lactotransferrin (LTF), TNF-, IL-1, cyclic adenosine monophosphate (cAMP), dopamine (DA), 5-hydroxytryptamine (5-HT), and mannan-binding lectin serine protease 2 (MASP2) in serum of patients or mice