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Free NO radical was generated by incubation of 2-( N , N -diethylamino)-diazenolate-2-oxide diethylammonium salt (DEA/NO) at the indicated concentrations in 1 ml of 50 mM TEA/HCl buffer (pH 7.4), or the respective extract at 37 C and monitored using LabChart 6 Software (ADInstruments, Version 6.1.3)

She said that according to the National Family Health Survey 2015-16 (NFHS-4) conducted by ministry of health and family welfare, the tobacco use among men in Punjab state declined from 33.8% (NFHS-3) to 19.2% (NFHS-4) and in women from 0.8% to 0.1% during last 10 years which is lowest among all the states of the country

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