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Mitochondrial DNA content determination by quantitative real-time polymerase chain reaction Real-time polymerase chain reaction (PCR) was performed to determine the total amount of mtDNA copy number of every single oocyte in all study groups

Animal studies administering 5-Amino-1MQ orally to diet-induced obese mice have shown dose-dependent reductions in body weight (typically 10-30% reduction compared to vehicle-treated obese controls over 4-12 week treatment periods), with the weight loss primarily reflecting fat mass reduction while lean mass is preserved or even increased

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