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84.97 54.16 in the control group), yet the diameters of umbilical vessels are reduced (Milnerowicz-Nabzdyk and Bizon, In vitro Techniques for Studying Nicotine Effects Endothelium-Dependent Vessel Relaxation Endothelium-dependent vessel relaxation is mediated by nitric oxide (NO), which is synthesized from the amino acid L-arginine by eNOS (Chechowska et al., Stem Cell Technology The rapid development of stem cell technology brings new opportunities for nicotine mechanism studies to facilitate precise treatment (Takahashi and Yamanaka, 2+ homeostasis due to nicotine exposure was recently demonstrated by using human embryonic stem cells (hESCs