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6 Limitation and future research priorities Despite the promising potential of ferroptosis-targeting therapies, several limitations currently hinder their translation into clinical practice

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Compared with control cells, the mRNAs of ACC (acetyl-CoA carboxylase), CHOP (CCAAT/enhancer-binding protein homologous protein) and caspase -3 significantly increased and PPAR - (peroxisome proliferator activated receptor ) mRNAs significantly decreased in the alcohol-induced model groups of human normal liver cell line LO2 (Figure 7A), hepatocellular carcinoma cell line HepG2 (Figure 7B) and model animal (Figure 7C)