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F., Ru, J

Key Functions of Vitamin C: Immune System Support : Enhances the activity of immune cells and helps fend off infections

Not to say all of these other things aren't helpful, but I believe a lot of those things come after this basic protocol, which then allows them to work so much better rather than fighting the inflammation that is being triggered by all this other stuff

2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added