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Take it for veterinary evaluation and give vitamin b12 injection for dogs according to instructions given by the vet

Treatment Overview & Aftercare Treatment duration: 30 minutes About Vitamin B12 Injections Vitamin B12 injections are a safe and effective way to replenish your B12 levels During the procedure Area cleansing at injection site Injection given into chosen area Before your appointment Avoid alcohol 24 hours before Increase water intake before treatment for optimum results Inform of any changes in medications or conditions Procedure details An injection is usually given into your upper arm, but your practitioner may choose a different site depending on your needs Duration of effect Our suggestion is to have one Vitamin B12 injection at weekly intervals, up to four doses (so four doses over four weeks)

p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells

Consistency in monitoring prevents recurrence of deficiency