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While healthy, metabolically active cells exclude the dye, damaged or dying cells retain it and become visibly stained, typically appearing red or pink under microscopic observation or on agar plates

Because angiogenesis is a defining hallmark of tumor growth and metastatic progression, any therapeutic agent that enhances neovascularization could, in theory, facilitate tumor expansion and promote metastatic dissemination

The studies mentioned above propose the possibility of the involvement of HO-1 in the control of both diabetes and its related complications